The Effect of Antibiotic Treatment and Gene Expression of Mex B Efflux Transporters on the Resistance in Pseudomonas Aeruginosa Biofilms

Pseudomonas aeruginosa, a Gram-negative, rod-shaped bacterium, holds prominent position as an antibiotic-resistant priority pathogen, according to the World Health Organization. Particularly prevalent in healthcare settings, this bacterium acts opportunistic causing nosocomial infections. The significant antibiotic resistance observed P. aeruginosa is multifactorial, encompassing intrinsic, acquired, and adaptive mechanisms. present study aims explore specific RND-type efflux pump genes implicated acquisition of resistances during transition PAO1 from its planktonic state more formidable resistant biofilm form. This investigation centered on MexB, aeruginosa. Our research focused highly component characterized by broad substrate specificity, primary function pump, substantial expression levels, notable clinical implications. Considering MexB’s critical role expelling various clinically relevant antimicrobial agents contribution multidrug resistance, our evaluate comparative efficacy three distinct categories, namely, Ofloxacin (OFX), Tobramycin (TOB), Ceftazidime (CAZ), regulating levels identified associated with biofilm’s ability remove antibiotics bacterial cells. Expression analysis transporter was performed isolating total RNA both samples, untreated treated (CAZ). Real-time quantitative reverse transcriptase PCR employed investigate changes MexA, MexX, MexY, OprM, RPSL collected samples. In absence treatment, MexB gene exhibited higher compared other state, supporting involvement when active. To further cultured forms while simultaneously treating them TOB, OFX, CAZ. Among employed, OFX demonstrated superior inhibiting growth biofilms downregulating Mex B thereby enhancing susceptibility OFX. TOB yielded comparable outcomes albeit slightly lesser extent reduction. Conversely, CAZ ineffectiveness reducing organism, rendering it incapable eradicating pathogen.